Antibody detection of SARS-CoV spike and nucleocapsid protein.
Identifieur interne : 005462 ( Main/Exploration ); précédent : 005461; suivant : 005463Antibody detection of SARS-CoV spike and nucleocapsid protein.
Auteurs : Mau-Sun Chang [Taïwan] ; Yen-Ta Lu ; Shin-Tsung Ho ; Chao-Chih Wu ; Tsai-Yin Wei ; Chia-Ju Chen ; Yun-Ting Hsu ; Po-Chen Chu ; Ching-Hsin Chen ; Jien-Ming Chu ; Ya-Lin Jan ; Chia-Chien Hung ; Chi-Chen Fan ; Yuh-Cheng YangSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Données de séquences moléculaires, Fractions subcellulaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (immunologie), Humains, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (), Protéines nucléocapside (immunologie), Séquence d'acides aminés, Technique d'immunofluorescence, Technique de Western, Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Données de séquences moléculaires, Fractions subcellulaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Protéines de l'enveloppe virale, Protéines nucléocapside, Séquence d'acides aminés, Technique d'immunofluorescence, Technique de Western.
English descriptors
- KwdEn :
- Amino Acid Sequence, Blotting, Western, Fluorescent Antibody Technique, Humans, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (immunology), Molecular Sequence Data, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (immunology), SARS Virus (immunology), SARS Virus (isolation & purification), Spike Glycoprotein, Coronavirus, Subcellular Fractions, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (immunology).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins.
- immunology : SARS Virus.
- isolation & purification : SARS Virus.
- Amino Acid Sequence, Blotting, Western, Fluorescent Antibody Technique, Humans, Molecular Sequence Data, Spike Glycoprotein, Coronavirus, Subcellular Fractions.
Abstract
Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6x His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV.
DOI: 10.1016/j.bbrc.2003.12.195
PubMed: 14751221
Affiliations:
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Le document en format XML
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l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Blotting, Western</term><term>Fluorescent Antibody Technique</term><term>Humans</term><term>Molecular Sequence Data</term><term>Spike Glycoprotein, Coronavirus</term><term>Subcellular Fractions</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Fractions subcellulaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Séquence d'acides aminés</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6x His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Chen, Chia Ju" sort="Chen, Chia Ju" uniqKey="Chen C" first="Chia-Ju" last="Chen">Chia-Ju Chen</name><name sortKey="Chen, Ching Hsin" sort="Chen, Ching Hsin" uniqKey="Chen C" first="Ching-Hsin" last="Chen">Ching-Hsin Chen</name><name sortKey="Chu, Jien Ming" sort="Chu, Jien Ming" uniqKey="Chu J" first="Jien-Ming" last="Chu">Jien-Ming Chu</name><name sortKey="Chu, Po Chen" sort="Chu, Po Chen" uniqKey="Chu P" first="Po-Chen" last="Chu">Po-Chen Chu</name><name sortKey="Fan, Chi Chen" sort="Fan, Chi Chen" uniqKey="Fan C" first="Chi-Chen" last="Fan">Chi-Chen Fan</name><name sortKey="Ho, Shin Tsung" sort="Ho, Shin Tsung" uniqKey="Ho S" first="Shin-Tsung" last="Ho">Shin-Tsung Ho</name><name sortKey="Hsu, Yun Ting" sort="Hsu, Yun Ting" uniqKey="Hsu Y" first="Yun-Ting" last="Hsu">Yun-Ting Hsu</name><name sortKey="Hung, Chia Chien" sort="Hung, Chia Chien" uniqKey="Hung C" first="Chia-Chien" last="Hung">Chia-Chien Hung</name><name sortKey="Jan, Ya Lin" sort="Jan, Ya Lin" uniqKey="Jan Y" first="Ya-Lin" last="Jan">Ya-Lin Jan</name><name sortKey="Lu, Yen Ta" sort="Lu, Yen Ta" uniqKey="Lu Y" first="Yen-Ta" last="Lu">Yen-Ta Lu</name><name sortKey="Wei, Tsai Yin" sort="Wei, Tsai Yin" uniqKey="Wei T" first="Tsai-Yin" last="Wei">Tsai-Yin Wei</name><name sortKey="Wu, Chao Chih" sort="Wu, Chao Chih" uniqKey="Wu C" first="Chao-Chih" last="Wu">Chao-Chih Wu</name><name sortKey="Yang, Yuh Cheng" sort="Yang, Yuh Cheng" uniqKey="Yang Y" first="Yuh-Cheng" last="Yang">Yuh-Cheng Yang</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Chang, Mau Sun" sort="Chang, Mau Sun" uniqKey="Chang M" first="Mau-Sun" last="Chang">Mau-Sun Chang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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